LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2273..2369) /label=attL2 /note="attL2 (c)" misc_signal complement(2384..2403) /note="T7 Promoter/priming site (c)" primer_bind 2411..2427 /note="M13 Reverse priming site" gene 2540..3349 /note="Kanamycin resistance gene" rep_origin 3470..4143 /note="pUC origin" PCR_frame 669..2273 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2293) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2290) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2281..4146,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2251..2271 /note="TEV site" source 673..2250 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2840 IMAGE:2966827" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2250 /gene="ALG8" /db_xref="GeneID:79053" /db_xref="HGNC:23161" /db_xref="MIM:608103" CDS 673..2250 /dnas_title="asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /gene="ALG8" /codon_start=1 /product="asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /protein_id="AAH01133.1" /db_xref="GI:12654595" /db_xref="GeneID:79053" /db_xref="HGNC:23161" /db_xref="MIM:608103" /translation="MAALTIATGTGNWFSALALGVTLLKCLLIPTYHSTDFEVHRNWL AITHSLPISQWYYEATSEWTLDYPPFFAWFEYILSHVAKYFDQEMLNVHNLNYSSSRT LLFQRFSVIFMDVLFVYAVRECCKCIDGKKVGKELTEKPKFILSVLLLWNFGLLIVDH IHFQYNGFLFGLMLLSIARLFQKRHMEGAFLFAVLLHFKHIYLYVAPAYGVYLLRSYC FTANKPDGSIRWKSFSFVRVISLGLVVFLVSALSLGPFLALNQLPQVFSRLFPFKRGL CHAYWAPNFWALYNALDKVLSVIGLKLKFLDPNNIPKASMTSGLVQQFQHTVLPSVTP LATLICTLIAILPSIFCLWFKPQGPRGFLRCLTLCALSSFMFGWHVHEKAILLAILPM SLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELPIKILLMLLFTIYSISSLKTLFR KEKPLFNWMETFYLLGLGPLEVCCEFVFPFTSWKVKYPFIPLLLTSVYCAVGVTYAWF KLYVSVLIDSAIGKTKKQ" misc_feature 673..2250 /note="ALG8 coding region" misc_difference 2179..2179 /gene="ALG8" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcggc gctcacaatt gccacgggta ctggcaattg gttttcggct 721 ttggcgctcg gggtgactct tctcaaatgc cttctcatcc ccacatacca ttccacagat 781 tttgaagtac accgaaactg gcttgctatc actcacagtt tgccaatatc acagtggtat 841 tatgaggcaa cttcagagtg gacgttggat tacccccctt tctttgcatg gtttgagtat 901 atcctgtcac atgttgccaa atattttgat caagaaatgc tgaatgtcca taatttgaat 961 tactccagct caaggacctt acttttccag agattttccg tcatctttat ggatgtactc 1021 tttgtgtatg ctgtccgtga gtgctgtaaa tgcattgatg gaaaaaaagt gggtaaagaa 1081 cttacagaaa agccaaaatt tattctgtcg gtattacttc tgtggaactt cgggttatta 1141 attgtggacc atattcattt tcagtacaat ggctttttat ttggattaat gctactctcc 1201 attgcacgat tatttcagaa aaggcatatg gaaggagcat ttctctttgc tgttctccta 1261 catttcaagc atatctacct ctatgtagca ccagcttatg gtgtatatct gctgcgatcc 1321 tactgtttca ctgcaaataa accagatggg tctattcgat ggaagagttt cagctttgtt 1381 cgtgttattt ccctgggact ggttgttttc ttagtttctg ctctttcatt gggtcctttc 1441 ctggccttga atcagctgcc tcaagtcttt tcccgactct ttcctttcaa gaggggcctc 1501 tgtcatgcat attgggctcc aaacttctgg gctttgtaca atgctttgga caaagtgctg 1561 tctgtcatcg gtttgaaatt gaaatttctt gatcccaaca atattcccaa ggcctcaatg 1621 acaagtggtt tggttcagca gttccaacac acagtccttc cctcagtgac tcccttggca 1681 accctcatct gcacactgat tgccatattg ccctctattt tctgtctttg gtttaaaccc 1741 caagggccca gaggctttct ccgatgtcta actctttgtg ccttgagctc ctttatgttt 1801 gggtggcatg ttcatgaaaa agccatactt ctagcaattc tcccaatgag ccttttgtct 1861 gtgggaaaag caggagacgc ttcgattttt ctgattctga ccacaacagg acattattcc 1921 ctctttcctc tgctcttcac tgcaccagaa cttcccatta aaatcttact catgttacta 1981 ttcaccatat atagtatttc gtcactgaag actttattca gaaaagaaaa acctcttttt 2041 aattggatgg aaactttcta cctgcttggc ctggggcctc tggaagtctg ctgtgaattt 2101 gtattccctt tcacctcctg gaaggtgaag taccccttca tccctttgtt actaacctca 2161 gtgtattgtg cagtaggcgt cacatatgct tggttcaaac tgtatgtttc agtattgatt 2221 gactctgcta ttggcaagac aaagaaacaa gaaaacttgt actttcaagg ctCCAGCTTT 2281 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 2341 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 2401 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 2461 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 2521 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 2581 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 2641 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 2701 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 2761 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 2821 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 2881 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 2941 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 3001 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 3061 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 3121 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 3181 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 3241 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 3301 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 3361 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 3421 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 3481 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 3541 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 3601 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 3661 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 3721 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 3781 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 3841 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 3901 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 3961 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 4021 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 4081 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 4141 CATGTT //